The characteristic time of glucose diffusion measured for muscle tissue at optical clearing

dc.contributor.author Oliveira,LM en
dc.contributor.author Maria Inês Carvalho en
dc.contributor.author Nogueira,EM en
dc.contributor.author Tuchin,VV en
dc.date.accessioned 2018-01-15T18:36:37Z
dc.date.available 2018-01-15T18:36:37Z
dc.date.issued 2013 en
dc.description.abstract The study of agent diffusion in biological tissues is very important to understand and characterize the optical clearing effects and mechanisms involved: tissue dehydration and refractive index matching. From measurements made to study the optical clearing, it is obvious that light scattering is reduced and that the optical properties of the tissue are controlled in the process. On the other hand, optical measurements do not allow direct determination of the diffusion properties of the agent in the tissue and some calculations are necessary to estimate those properties. This fact is imposed by the occurrence of two fluxes at optical clearing: water typically directed out of and agent directed into the tissue. When the water content in the immersion solution is approximately the same as the free water content of the tissue, a balance is established for water and the agent flux dominates. To prove this concept experimentally, we have measured the collimated transmittance of skeletal muscle samples under treatment with aqueous solutions containing different concentrations of glucose. After estimating the mean diffusion time values for each of the treatments we have represented those values as a function of glucose concentration in solution. Such a representation presents a maximum diffusion time for a water content in solution equal to the tissue free water content. Such a maximum represents the real diffusion time of glucose in the muscle and with this value we could calculate the corresponding diffusion coefficient. en
dc.identifier.uri http://repositorio.inesctec.pt/handle/123456789/6221
dc.identifier.uri http://dx.doi.org/10.1088/1054-660x/23/7/075606 en
dc.language eng en
dc.relation 5135 en
dc.rights info:eu-repo/semantics/openAccess en
dc.title The characteristic time of glucose diffusion measured for muscle tissue at optical clearing en
dc.type article en
dc.type Publication en
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