Neurondynamics: A method for neurotransmitter vesicle movement characterization in neurons
Neurondynamics: A method for neurotransmitter vesicle movement characterization in neurons
dc.contributor.author | Carpinteiro,FA | en |
dc.contributor.author | Costa,PM | en |
dc.contributor.author | Espinoza,MS | en |
dc.contributor.author | Silva,IM | en |
dc.contributor.author | João Paulo Cunha | en |
dc.date.accessioned | 2018-01-16T17:06:51Z | |
dc.date.available | 2018-01-16T17:06:51Z | |
dc.date.issued | 2014 | en |
dc.description.abstract | Automated tracking of axonal neurotransmitter vesicles is a challenging problem in neuroscience. The present vesicle tracking is typically performed manually over confocal microscopy images. Neuron Dynamics is a method designed to automate and speed-up the characterization of global vesicle movement in neurons while yielding high accuracy and precision results (similar or better than expert clinicians). For a set of fluorescent-marked vesicles "films", Neuron-Dynamics performs a two stage approach: 1) Training: the system asks the user to mark a set of vesicles and the position of the cellular body; 2) Detection & tracking: based on the previous training, the system runs a Bayesian classifier over the image sequence to detect and classify vesicles and their movements (speed and direction). The obtained results were compared to another state-of-the-art method (FluoTracker), and were found greatly higher in accuracy, sensitivity, specificity and precision. Although Neuron Dynamics is a semi-automated process, it is significantly faster than manual tracking and can be adapted to be used for similar approaches for other biological samples. © 2014 IEEE. | en |
dc.identifier.uri | http://repositorio.inesctec.pt/handle/123456789/6477 | |
dc.identifier.uri | http://dx.doi.org/10.1109/isbi.2014.6867913 | en |
dc.language | eng | en |
dc.relation | 5864 | en |
dc.rights | info:eu-repo/semantics/embargoedAccess | en |
dc.title | Neurondynamics: A method for neurotransmitter vesicle movement characterization in neurons | en |
dc.type | conferenceObject | en |
dc.type | Publication | en |
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