Please use this identifier to cite or link to this item: http://repositorio.inesctec.pt/handle/123456789/3644
Title: Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli outer membrane proteins
Authors: Queiros,RB
Carlos Jesus Gouveia
José Ramiro Fernandes
Pedro Jorge
Issue Date: 2014
Abstract: An evanescent wave fiber optic sensor for detection of Escherichia coli (E. coli) outer membranes proteins (EcOMPs) using long period gratings (LPGs) as a refractometric platform is presented. The sensing probes were attained by the functionalization of LPGs inscribed in single mode fiber using two different methods of immobilization; electrostatic assembly and covalent binding. The resulting label-free configuration enabled the specific recognition of EcOMPs in water by monitoring the resonance wavelength shift due to refractive index changes induced by binding events. The sensors displayed linear responses in the range of 0.1 nM to 10 nM EcOMPs with sensitivities of -0.1563 +/- 0.005 nm decade(-1) [EcOMP, M] (electrostatic method) and -0.1597 +/- 0.004 nm decade(-1) [EcOMP, M] (covalent method). The devices could be regenerated (under low pH conditions) with a deviation less than 0.1% for at least three subsequent detection events. The sensors were also applied to spiked environmental water samples.
URI: http://repositorio.inesctec.pt/handle/123456789/3644
http://dx.doi.org/10.1016/j.bios.2014.06.062
metadata.dc.type: article
Publication
Appears in Collections:CAP - Articles in International Journals

Files in This Item:
File Description SizeFormat 
P-009-PV4.pdf2.14 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.