Please use this identifier to cite or link to this item: http://repositorio.inesctec.pt/handle/123456789/7343
Title: Biosensor for label-free DNA quantification based on functionalized LPGs
Authors: Goncalves,HMR
Moreira,L
Pereira,L
Pedro Jorge
Gouveia,C
Martins Lopes,P
José Ramiro Fernandes
Issue Date: 2016
Abstract: A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
URI: http://repositorio.inesctec.pt/handle/123456789/7343
http://dx.doi.org/10.1016/j.bios.2015.10.001
metadata.dc.type: article
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Appears in Collections:Non INESC TEC publications - Articles in International Journals

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