Non INESC TEC publications - Indexed Articles in Journals

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    High sensitivity LPG Mach-Zehnder sensor for real-time fuel conformity analysis
    ( 2013) Osorio,JH ; Mosquera,L ; Carlos Jesus Gouveia ; Biazoli,CR ; Hayashi,JG ; Pedro Jorge ; Cordeiro,CMB
    A high sensitivity refractive index sensor based on the combination of mechanically induced long period gratings (LPG) and fiber tapers was developed for real-time fuel quality analysis. The sensor was built in a Mach-Zehnder configuration by employing a pair of in-series gratings. In order to enhance sensor sensitivity, the region between both LPGs was tapered down from 125 to 10 mu m. The system was tested by measuring water concentration in ethanol and ethanol concentration in commercial gasoline. The tapered sensor has shown an average sensitivity of 930 nm/RIU, 18 times higher than the non-tapered version. The resolution limit of the system using spectral interrogation was estimated to be 0.06% of ethanol dissolved in gasoline. For the purpose of real-time monitoring, an interrogation system based on white light interferometry (WLI) and virtual instrumentation was employed to evaluate ethanol evaporation in water, avoiding the use of spectral analysis. The WLI system, using phase tracking techniques, enabled us to record the evolution of the ethanol concentration in water with a resolution of 0.005% (v/v).
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    A new Branch and Bound method for a discrete truss topology design problem
    ( 2013) Adelaide Cerveira ; Agra,A ; Bastos,F ; Gromicho,J
    Our paper considers a classic problem in the field of Truss Topology Design, the goal of which is to determine the stiffest truss, under a given load, with a bound on the total volume and discrete requirements in the cross-sectional areas of the bars. To solve this problem we propose a new two-stage Branch and Bound algorithm. In the first stage we perform a Branch and Bound algorithm on the nodes of the structure. This is based on the following dichotomy study: either a node is in the final structure or not. In the second stage, a Branch and Bound on the bar areas is conducted. The existence or otherwise of a node in this structure is ensured by adding constraints on the cross-sectional areas of its incident bars. In practice, for reasons of stability, free bars linked at free nodes should be avoided. Therefore, if a node exists in the structure, then there must be at least two incident bars on it, unless it is a supported node. Thus, a new constraint is added, which lower bounds the sum of the cross-sectional areas of bars incident to the node. Otherwise, if a free node does not belong to the final structure, then all the bar area variables corresponding to bars incident to this node may be set to zero. These constraints are added during the first stage and lead to a tight model. We report the computational experiments conducted to test the effectiveness of this two-stage approach, enhanced by the rule to prevent free bars, as compared to a classical Branch and Bound algorithm, where branching is only performed on the bar areas.
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    Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
    ( 2013) Arantes Rodrigues,R ; Pinto Leite,R ; Lio Gonçalves ; Palmeira,C ; Santos,L ; Colaco,A ; Oliveira,P
    The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with cisplatin (3, 6, 13, and 18 mu M) and sunitinib malate (1, 2, 4, 6, and 20 mu M), either in isolation or combined, over the course of 72 hours. 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. The combination index (CI) was calculated based on the Chou and Talalay method. In isolation, cisplatin and sunitinib malate statistically (p < 0.05) decrease cell viability in all cell lines in a dose-dependent manner, with the presence of autophagic vacuoles. A cell cycle arrest in early S-phase and in G(0)/G(1) -phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. Treatment of urinary bladder-cancer cells with a combination of cisplatin and sunitinib malate showed a synergistic effect (CI < 1). Autophagy and apoptosis studies showed a greater incidence when the combined treatment was put into use. This hints at the possibility of a new combined therapeutic approach. If confirmed in vivo, this conjugation may provide a means of new perspectives in muscle-invasive urinary bladder cancer treatment.
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    Interrogation Sensing Scheme Based on a Figure-of-Eight Fiber Loop Mirror
    ( 2013) Silva,RM ; Layeghi,A ; Zibaii,MI ; Latifi,H ; José Luís Santos ; Lobo Ribeiro,ABL ; Orlando Frazão
    A fiber optic interrogation sensor scheme based on a "figure-of-eight" configuration created from a single directional 3 x 3 fiber optic coupler is proposed. Two loops are formed in each arm and one of them contains the sensing head and the other is used as reference signal. A theoretical study based on Jones matrix analysis of this fiber loop mirror combination is reported. The optical configuration is tested as an interrogation scheme for a fiber strain sensor where the spectral response arises from the combination of the reference signal modulated by the sensor signal. The strain sensor configuration shows a phase sensitivity of 6.7 +/- 4.38 x 10(-2) mrad/mu strain by linear regression.
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    Fiber laser sensor based on a phase-shifted chirped grating for acoustic sensing of partial discharges
    ( 2013) Lima,SEU ; Farias,RG ; Araujo,FM ; Ferreira,LA ; José Luís Santos ; Vladimiro Miranda ; Orlando Frazão
    Acoustic emission monitoring is often used in the diagnosis of electrical and mechanical incipient faults in the high voltage apparatus. Partial discharges are a major source of insulation failure in electric power transformers, and the differentiation from other sources of acoustic emission is of the utmost importance. This paper reports the development of a new sensor concept - a fiber laser sensor based on a phase-shifted chirped fiber grating - for the acoustic emission detection of incipient faults in oil-filled power transformers. These sensors can be placed in the inner surface of the transformer tank wall, not affecting the insulation integrity of the structure and improving fault detection and location. The performance of the sensing head is characterized and compared for different surrounding media: air, water, and oil. The results obtained indicate the feasibility of this sensing approach for the industrial development of practical solutions. © 2012 The Author(s).
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    New design for temperature-strain discrimination using fiber Bragg gratings embedded in laminated composites
    ( 2013) Rodriguez Cobo,L ; Marques,AT ; Lopez Higuera,JM ; José Luís Santos ; Orlando Frazão
    A new smart structure based on fiber Bragg gratings (FBGs) embedded into composite laminates for temperature and strain simultaneous measurement has been designed and experimentally tested. Two holes have been drilled at preset locations in the composite plate to create different strain sensitivities at different locations. The proposed design has been compared to three reference sensing heads also based on embedding FBGs into composite materials. Experimental results agree remarkably well with mechanical simulations and validate all the tested designs for the temperature-strain discrimination. Based on the same principle, another sensing head with a long single FBG embedded has also been designed and experimentally tested, obtaining temperature independent strain measurement.
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    Computational models for new fiber optic tweezers
    ( 2013) Ana Rita Ribeiro ; Pedro Jorge ; Ariel Guerreiro
    This paper discusses the calculation of the trapping forces in optical tweezers using a combination of the finite differences time domain (FDTD) method and the Lorentz force on electric dipoles. The results of 2D simulations of the trapping of a circular particle by a waveguide with a circular tip are presented and discussed. © 2012 The Author(s).
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    E-cadherin and adherens-junctions stability in gastric carcinoma: Functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and V
    ( 2013) Pinho,SS ; Figueiredo,J ; Cabral,J ; Carvalho,S ; Dourado,J ; Magalhaes,A ; Gaertner,F ; Ana Maria Mendonça ; Isaji,T ; Cu,JG ; Carneiro,F ; Seruca,R ; Taniguchi,N ; Reis,CA
    Background: E-cadherin is a cell-cell adhesion molecule and the dysfunction of which is a common feature of more than 70% of all invasive carcinomas, including gastric cancer. Mechanisms behind the loss of E-cadherin function in gastric carcinomas include mutations and silencing at either the DNA or RNA level. Nevertheless, in a high percentage of gastric carcinoma cases displaying E-cadherin dysfunction, the mechanism responsible for E-cadherin dysregulation is unknown. We have previously demonstrated the existence of a bi-directional cross-talk between E-cadherin and two major N-glycan processing enzymes, N-acetylglucosaminyltransferase-III or -V (GnT-III or GnT-V). Methods: In the present study, we have characterized the functional implications of the N-glycans catalyzed by GnT-III and GnT-V on the regulation of E-cadherin biological functions and in the molecular assembly and stability of adherens-junctions in a gastric cancer model. The results were validated in human gastric carcinoma samples. Results: We demonstrated that GnT-III induced a stabilizing effect on E-cadherin at the cell membrane by inducing a delay in the turnover rate of the protein, contributing for the formation of stable and functional adherens-junctions, and further preventing clathrin-dependent E-cadherin endocytosis. Conversely, GnT-V promotes the destabilization of E-cadherin, leading to its mislocalization and unstable adherens-junctions with impairment of cell-cell adhesion. Conclusions: This supports the role of GnT-III on E-cadherin-mediated tumor suppression, and GnT-V on E-cadherin-mediated tumor invasion. General significance: These results contribute to fill the gap of knowledge of those human carcinoma cases harboring E-cadherin dysfunction, opening new insights into the molecular mechanisms underlying E-cadherin regulation in gastric cancer with potential translational clinical applications.
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    Next generation of Fabry-Perot sensors for high-temperature
    ( 2013) Marta Sofia Ferreira ; Paulo Roriz Oliveira ; Susana Oliveira Silva ; José Luís Santos ; Orlando Frazão
    This paper presents an overview of optical fiber sensors based on Fabry-Perot interferometers with a focus on high temperature applications. The next generation of these fiber types interferometers are based in photonic crystal fibers, microfabrication as well as by chemical etching of special structures. High temperature measurements with linear behavior are observed namely in un-doped fibers, i.e., with a pure silica composition. Three new configurations are presented as possible solutions to be considered in extreme conditions.
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    Exciting the optical response of nanowire metamaterial films on the tip of optical fibres
    ( 2013) Leite,IT ; Hierro Rodriguez,A ; Silva,AO ; Teixeira,JM ; Sousa,CT ; Fernandez Garcia,MP ; Araujo,JP ; Pedro Jorge ; Ariel Guerreiro
    This Letter reports on the assembly on the tip of an optical fibre of a metamaterial film fabricated by a self-assembly bottom-up method, composed of silver nanowires embedded in an alumina matrix. By illuminating the film through the fibre in a reflection configuration, we observe experimentally the optical response of the metamaterial in agreement with theoretical predictions and interpreted as the excitation of surface plasmon-polaritons in the cylindrical surface of the nanowires. These results pave the way for low-cost optical fibre devices that incorporate metamaterial films. (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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    Application of Nanotechnology in the Agro-Food Sector
    ( 2013) Lopes,CM ; José Ramiro Fernandes ; Martins Lopes,P
    Nanotechnology is an emerging field of research that has been widely applied in different scientific and engineering areas. The agro-food sector is not an exception, which considers its applicability in several areas of major interest for both consumers and producers. This review considers major concepts related to nanostructures and nano-based instruments used in the food sector, as well as their applications in agro-food products. Food safety through the use of nanosensors for pathogen detection, smart packaging, and valorisation of food products by nanoencapsulation/nanodelivery of food ingredients (e.g. flavours) are examples of important areas of nanotechnology. Consumers' apprehension regarding food stability and safety issues is also considered.
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    High-speed integrated optical logic based on the protein bacteriorhodopsin
    ( 2013) Mathesz,A ; Fabian,L ; Valkai,S ; Daniel Alexandre ; Paulo Vicente Marques ; Ormos,P ; Wolff,EK ; Der,A
    The principle of all-optical logical operations utilizing the unique nonlinear optical properties of a protein was demonstrated by a logic gate constructed from an integrated optical Mach-Zehnder interferometer as a passive structure, covered by a bacteriorhodopsin (bR) adlayer as the active element. Logical operations were based on a reversible change of the refractive index of the bR adlayer over one or both arms of the interferometer. Depending on the operating point of the interferometer, we demonstrated binary and ternary logical modes of operation. Using an ultrafast transition of the bR photocycle (BR-K), we achieved high-speed (nanosecond) logical switching. This is the fastest operation of a protein-based integrated optical logic gate that has been demonstrated so far. The results are expected to have important implications for finding novel, alternative solutions in all-optical data processing research.
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    Strong birefringence tuning of optical waveguides with femtosecond laser irradiation of bulk fused silica and single mode fibers
    ( 2013) Fernandes,LA ; Grenier,JR ; Paulo Vicente Marques ; Stewart Aitchison,J ; Herman,PR
    Birefringence tunability is demonstrated in waveguides formed in bulk fused silica and in the core of single mode fibers, by femtosecond laser writing of stress inducing tracks that are placed with different geometries around the core of the waveguides. The femtosecond laser generated stress effect was probed by the birefringence induced spectral splitting of either Bragg grating waveguides in bulk fused silica or weakly modulated, femtosecond laser induced Bragg gratings in optical fibers. Birefringence values as low as 4 × 10 -6 and up to 2 × 10-3 were obtained by controlling the fabrication conditions such as the laser pulse energy, the writing femtosecond laser polarization, the number of overwriting exposures, and the geometry of the induced stress tracks. Wave retarders are developed and characterized by a cross polarization technique to provide the spectral response of the stress induced birefringence, offering the convenient fabrication of short length and broadband in-line polarization devices. With this approach, millimeter length tracks provided 10 nm bandwidth polarization retarders in a single mode fiber and a 65 nm bandwidth retarders in bulk fused silica. © 1983-2012 IEEE.
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    Oxidation of ZnO thin films during pulsed laser deposition process
    ( 2013) De Posada,E ; Moreira,L ; Javier Cruz ; Arronte,M ; Ponce,LV ; Flores,T ; Lunney,JG
    Pulsed laser deposition of ZnO thin films, using KrF laser, is analysed. The films were deposited on (001) sapphire substrates at 400 A degrees C, at two different oxygen pressures (0 center dot 3 and 0 center dot 4 mbar) and two different target-substrate distances (30 and 40 mm). It is observed that in order to obtain good quality in the photoluminescence of the films, associated with oxygen stoichiometry, it is needed to maximize the time during which the plasma remains in contact with the growing film (plasma residence time), which is achieved by selecting suitable combinations of oxygen pressures and target to substrate distances. It is also discussed that for the growth parameters used, the higher probability for ZnO films growth results from the oxidation of Zn deposited on the substrate and such process takes place during the time that the plasma is in contact with the substrate. Moreover, it is observed that maximizing the plasma residence time over the growing film reduces the rate of material deposition, favouring the surface diffusion of adatoms, which favours both Zn-O reaction and grain growth.
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    Induced polarized state in intentionally grown oxygen deficient KTaO3 thin films
    ( 2013) Mota,DA ; Romaguera Barcelay,Y ; Tkach,A ; Javier Cruz ; Vilarinho,PM ; Tavares,PB ; Agostinho Moreira,JA ; Almeida,A
    Deliberately oxygen deficient potassium tantalate thin films were grown by RF magnetron sputtering on Si/SiO2/Ti/Pt substrates. Once they were structurally characterized, the effect of oxygen vacancies on their electric properties was addressed by measuring leakage currents, dielectric constant, electric polarization, and thermally stimulated depolarization currents. By using K2O rich KTaO3 targets and specific deposition conditions, KTaO3-delta oxygen deficient thin films with a K/Ta = 1 ratio were obtained. Room temperature X-ray diffraction patterns show that KTaO3-delta thin films are under a compressive strain of 2.3% relative to KTaO3 crystals. Leakage current results reveal the presence of a conductive mechanism, following the Poole-Frenkel formalism. Furthermore, dielectric, polarization, and depolarization current measurements yield the existence of a polarized state below T-pol similar to 367 degrees C. A Cole-Cole dipolar relaxation was also ascertained apparently due to oxygen vacancies induced dipoles. After thermal annealing the films in an oxygen atmosphere at a temperature above T-pol, the aforementioned polarized state is suppressed, associated with a drastic oxygen vacancies reduction emerging from annealing process. (C) 2013 AIP Publishing LLC.
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    On the improvement of strain measurements with FBG sensors embedded in unidirectional composites
    ( 2013) Pereira,G ; Frias,C ; Faria,H ; Orlando Frazão ; Marques,AT
    Optical fibre Bragg grating (FBG) sensors are now quite established and widely used in strain measurements of composites. However, insufficient understanding of the limitations of the embedment and measuring techniques often lead to inaccurate and inconclusive results. In this study, a novel method to improve the reliability and accuracy of the strain measurements on unidirectional composites using embedded FBG sensors was successfully developed. Using a carbon/epoxy prepreg system, test specimens were manufactured with longitudinally embedded FBG sensors. The combined behaviour of the sensors and the host material was characterized and a calibration rule (correction factor) was determined for the chosen material. The consistency of the results with both theoretical and empirical assumptions suggests that the proposed method is applicable to a wide range of FBG sensors and host materials.
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    Automatic Lane Segmentation in TLC Images Using the Continuous Wavelet Transform
    ( 2013) Moreira,B ; Sousa,A ; Ana Maria Mendonça ; Aurélio Campilho
    This paper describes a new methodology for lane detection in Thin-Layer Chromatography images. An approach based on the continuous wavelet transform is used to enhance the relevant lane information contained in the intensity profile obtained from image data projection. Lane detection proceeds in three phases: the first obtains a set of candidate lanes, which are validated or removed in the second phase; in the third phase, lane limits are calculated, and subtle lanes are recovered. The superior performance of the new solution was confirmed by a comparison with three other methodologies previously described in the literature.
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    A multiadaptive sampling technique for cost-effective network measurements
    ( 2013) João Marco ; Carvalho,P ; Lima,SR
    The deployment of efficient measurement solutions to assist network management tasks without interfering with normal network operation assumes a prominent role in today's high-speed networks attending to the huge amounts of traffic involved. From a myriad of proposals for traffic measurement, sampling techniques are particularly relevant contributing effectively for this purpose as only a subset of the overall traffic volume is handled for processing, preserving ideally the correct estimation of network statistical behavior. In this context, this paper proposes MuST - a multiadaptive sampling technique based on linear prediction, aiming at reducing significantly the measurement overhead and still assuring that traffic samples reflect the statistical characteristics of the global network traffic under analysis. Conversely to current sampling techniques, MuST is a multi and self-adaptive technique as both the sample size and interval between samples are self-adjustable parameters according to the ongoing network activity and the accuracy of prediction achieved. The tests carried out demonstrate that the proposed sampling technique is able to achieve accurate network estimations with reduced overhead, using throughput as reference parameter. The evaluation results, obtained resorting to real traffic traces representing wired and wireless aggregated traffic scenarios and actual network services, prove that the simplicity, flexibility and self-adaptability of the proposed technique can be successfully explored to improve network measurements efficiency over distinct traffic conditions. For optimization purposes, this paper also includes a study of the impact of varying the order of prediction, i.e., of considering different degrees of past memory in the self-adaptive estimation mechanism. The significance of the obtained results is demonstrated through statistical benchmarking.
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    Computational study of the covalent bonding of microcystins to cysteine residues - a reaction involved in the inhibition of the PPP family of protein phosphatases
    ( 2013) Susana Rodrigues Pereira ; Vasconcelos,VM ; Antunes,A
    Microcystins (MCs) are cyclic peptides, produced by cyanobacteria, that are hepatotoxic to mammals. The toxicity mechanism involves the potent inhibition of protein phosphatases, as the toxins bind the catalytic subunits of five enzymes of the phosphoprotein phosphatase (PPP) family of serine/threonine-specific phosphatases: Ppp1 (aka PP1), Ppp2 (aka PP2A), Ppp4, Ppp5 and Ppp6. The interaction with the proteins includes the formation of a covalent bond with a cysteine residue. Although this reaction seems to be accessory for the inhibition of PPP enzymes, it has been suggested to play an important part in the biological role of MCs and furthermore is involved in their nonenzymatic conjugation to glutathione. In this study, the molecular interaction of microcystins with their targeted PPP catalytic subunits is reviewed, including the relevance of the covalent bond for overall inhibition. The chemical reaction that leads to the formation of the covalent bond was evaluated in silico, both thermodynamically and kinetically, using quantum mechanical-based methods. As a result, it was confirmed to be a Michael-type addition, with simultaneous abstraction of the thiol hydrogen by a water molecule, transfer of hydrogen from the water to the alpha,beta-unsaturated carbonyl group of the microcystin and addition of the sulfur to the beta-carbon of the microcystin moiety. The calculated kinetics are in agreement with previous experimental results that had indicated the reaction to occur in a second step after a fast noncovalent interaction that inhibited the enzymes per se.